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KMID : 0379119950230040340
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Korean Journal of Mycology
1995 Volume.23 No. 4 p.340 ~ p.347
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Immuno - stimulating Polysaccharides from the Fruiting Bodies of Fomitella fraxinea (2)
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이재훈/Lee, Jae Hoon
김환묵/한상배/유익동/조수묵/유승헌/Kim, Hwan Mook/Han, Sang Bae/Yoo, Ick Dong/Cho, Soo Muk/Yu, Seung Hun
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Abstract
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Polysaccharide FHW was extracted from the fruiting bodies of Fomitella fraxinea with hot-water treatment and then fractionated into FHW-I and FHW-II on DEAF-Cellulose chromatography. FHW-I and FCW-U were further purified into FHW-Ia and Ib, FHW-Ha and Hb on gel permeation chromatography, respectively. A small amount of uronic acid was detected and glucose, galactose, fucose, and mannose were found to be main sugars in the polysaccharides. Protein was detected in FHW-Ia, FHW-IIa, and FHW-Hb, but not in FHW-Ib. FHW-Ia was identified to be a fuco-gluco-mannogalactan with molecular weight of 19,000 and FHW-Ib was a gluco-fuco-mannogalactan of 15,000. FHW-IIa and FHW-Hb were galacto-mannogiucan and their molecular weights were estimated to be 31,000 and 9,000, respectively. Both FHW-IIb and FHW-IIb did not show an absorption band characteristic of the β-glycosidic linkage in IR spectra. FHW-IIb showed a strong immuno-stimulating activity but the other three polysaccharides showed a weak activity.
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